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李雪玲
2016年06月03日 10:27      浏览:

 

李雪玲 博士
学科:动物基因工程及干细胞生物学
电话:+86-471-3679807
电子邮件:lixueling  @hotmail.com
通信地址:内蒙古呼和浩特市昭君路24号,内蒙古大学南校区国家动物转基因技术研究中心,邮编010070
简要介绍:

科研工作主要集中于动物基因修饰和基因敲除、人和小鼠胚胎干细胞基因操作和定向分化、家畜动物胚胎干细胞的分离和培养等方面。用经典的同源重组方法将绿色荧光蛋白(GFP)和红色荧光蛋白(RFP)基因定点整合到小鼠胚胎干细胞的胰-十二指肠同源盒1(pancreatic and duodenal homeobox 1,PDX1)基因和胰岛素(InsulinINS)基因位点,在体外定向分化过程中通过检测绿色荧光蛋白和红色荧光蛋白的表达以确定胚胎干细胞向胰岛β-细胞分化,用以鉴定胚胎干细胞定向分化的生长因子以及寻找胚胎干细胞向β-细胞定向分化的最佳方法,此研究结果发表于《Stem Cell Research》。首次在人的胚胎干细胞的胰岛素基因位点定点插入绿色荧光蛋白基因,而且在体外分化的β-细胞中都检测到绿色荧光蛋白的表达,证明绿色荧光蛋白忠实地代表了胰岛素基因的表达,可以用于代替胰岛素基因来分离和鉴定β-细胞,解决了免疫荧光检测的繁琐步骤,而且可以用流式细胞分离仪轻松分离β-细胞,进行进一步的生长成熟的研究,大大促进了胚胎干细胞对于糖尿病的治疗研究,此研究结果发表在《Diabetologia》。目前主要从事最新基因编辑技术应用于草食家畜的基因编辑,以及牛羊胚胎干细胞的建立方面的研究。

    本人或与他人合作在国际和国内科研杂志上发表学术论文20多篇,其中10多篇为SCI収录。
 教育经历
2003/1-2011/6,澳大利亚,  莫纳什大学,博士后
1998/9-2002/7,内蒙古大学,生命科学学院,生物系,博士
1992/9-1995/7,内蒙古大学生物系,硕士
1988/9-1992/7,内蒙古大学生物系,学士
 研究经历:
2012/8-现在,内蒙古大学,实验动物研究中心,教授
2005/3-2012/7,澳大利亚,莫纳什大学免疫和干细胞实验室,博士后研究员
2003/1-2005/2,澳大利亚,莫纳什大学繁殖和发育研究所,博士后研究员
1998/9-2002/7,内蒙古大学,生命科学学院,教师兼在职博士研究生
1995/7-1998/9,内蒙古大学,生命科学学院,教师兼科研
 正在承担科研项目:
1. 国家转基因重大专项“转基因新技术新方法”子课题,项目编号“2013ZX08010-001”;经费来源:农业部(115万元)。项目起止年月:2013.1.1-2015.12.30。子课题负责人。  
2. 国家自然科学基金面上项目“miR-375在人胚胎干细胞定向分化为beta-细胞过程中的作用及对其成熟的影响”,项目编号:81471001,经费来源:国家自然科学基金委员会(73万元)。项目起止年月:2015.1.1-2018.12.31。  
3. 国家自然科学基金地区项目“牛体细胞核移植(SCNT)类滋养层干细胞基因表达和分化形成双核滋养层细胞能力的研究”,项目编号:31460309,经费来源:国家自然科学基金委员会(50万元)。项目起止年月:2015.1.1-2018.12.31。  
 代表论著:
    Hou D, Su M, Li X, Li Z, Yun T, Zhao Y, Zhang M, Zhao L, Li R, Yu H, Li XL*. (2015) The Efficient Derivation of TrophoblastCells from Porcine In Vitro Fertilized andParthenogenetic Blastocysts and Culture with ROCKInhibitor Y-27632. PLoS ONE 10(11): e0142442.doi:10.1371/journal.pone.0142442.
    HanXJ, LiangH, Yun T, Zhao YH, ZhangML, ZhaoLH, Li RFand LiXL*. Decreased expression of humanized Fat-1 in porcine fetal fibroblasts following deletion of PGK-neomycin resistance.Genet. Mol. Res. 2015 Step, 14 (3): 11594-11604.
ZhaoLH, ZhaoYH,  LiangH,  YunT,  HanXJ,  ZhangML,  ZhouX,  HouDX,  Li RF, LiXL*. A promoter trap vector for knocking out bovine myostatin gene with high targeting efficiency.Genet. Mol. Res. 2015 Mar, 14 (1): 2750 – 2761.
   Huang X, Han X, Uyunbilig B, Zhang M, Duo S, Zuo YC, Zhao Y, Yun T, Tai D,  Wang C, LiJ, Li XL*, Li R. Establishment of Bovine Trophoblast Stem-Like Cells from in Vitro Produced Blastocyst-Stage Embryosusing 2  Inhibitors. Stem Cells Dev. 2014 Jul 1;23(13):1501-14.

    Li XL. MiR-375, a microRNA related to diabetes. Gene.  2014 Jan 1;533(1):1-4.

    M.Q. Liu, J.G. Wang and X.L. Li. Cloning and sequencing  of Wistar-Imamichi(W-I)rat growth hormone gene and genetic polymorphisms  analysis using PCR-SSCP technology. Genetics and Molecular Research.2013,  12(4):6203-6211.

    MQu X, Li XL, Zheng Y, Ren Y, Puelles VG, Caruana G,  Nikolic-Paterson DJ, Li J.Regulation of renal fibrosis by smad3 thr388  phosphorylation.Am J Pathol. 2014 Apr;184(4):944-52.

    Micallef SJ, Li XL, Schiesser JV, Hirst CE, Yu QC, Lim  SM, Nostro MC, Elliott DA, Sarangi F. , Harrison LC, Keller G, Elefanty AG,  Stanley EG. INSGFP/w human embryonic stem cells facilitate isolation of in vitro  derived insulin-producing cells. Diabetologia. 2012 Mar; 55(3):694-706.

    Basford CL, Prentice KJ, Hardy AB, Sarangi F, Micallef SJ, Li  XL, Guo Q, Elefanty AG, Stanley EG, Keller G, Allister EM, Nostro MC,  Wheeler MB. The functional and molecular characterisation of human embryonic  stem cell-derived insulin-positive cells compared with adult pancreatic beta  cells. Diabetologia. 2012 Feb;55(2):358-71.

     Elliott DA, Braam SR, Koutsis K, Ng ES, Jenny R, Lagerqvist EL, Biben C,  Hatzistavrou T, Hirst CE, Yu QC, Skelton RJ, Ward-van Oostwaard D, Lim SM,  Khammy O, Li XL, Hawes SM, Davis RP, Goulburn AL, Passier R,  Prall OW, Haynes JM, Pouton CW, Kaye DM, Mummery CL, Elefanty AG, Stanley EG.  NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and  cardiomyocytes. Nat Methods. 2011 Oct 23;8(12):1037-40.

    Lim SM, Li XL, Schiesser JV, Holland AM, Elefanty AG,  Stanley EG, Micallef S. Temporal restriction of pancreatic branching competence  during embryogenesis is mirrored in differentiating embryonic stem cells. Stem  Cells Dev. 2012 Jul 1; 21(10):1662-74.

     Nostro MC, Sarangi F, Ogawa S, Holtzinger A, Corneo B, Li  XL, Micallef SJ, Park IH, Basford C, Wheeler MB, Daley GQ, Elefanty AG,  Stanley EG, Keller G. Stage-specific signaling through TGFβ family members and  WNT regulates patterning and pancreatic specification of human pluripotent stem  cells. Development. 2011 Mar; 138(5):861-71.

    Davis RP, Costa M, Grandela C, Holland AM, Hatzistavrou T, Micallef  SJ, Li XL, Goulburn AL, Azzola L, Elefanty AG, Stanley EG “A  protocol for removal of antibiotic resistance cassettes from human embryonic  stem cells genetically modified by homologous recombination or transgenesis.”Nat  Protoc. 2008; 3(10):1550-8.

   Micallef SJ, Li XL, Elefanty AG, Stanley EG. “Pancreas  differentiation of mouse ES cells.” Curr Protoc Stem Cell Biol. 2007 Aug;Chapter  1:Unit 1G.2.

   Micallef SJ, Li XL, Janes ME, Jackson SA, Sutherland RM,  Lew AM, Harrison LC, Elefanty AG, Stanley EG. “Endocrine cells develop within  pancreatic bud-like structures derived from mouse ES cells differentiated in  response to BMP4 and retinoic acid.”Stem Cell Res. 2007 Oct; 1(1):25-36.

     Holland AM, Micallef SJ, Li XL, Elefanty AG, Stanley  EG. “A mouse carrying the green fluorescent protein gene targeted to the Pdx1  locus facilitates the study of pancreas development and function.”Genesis. 2006  Jun; 44(6):304-7.

    Li XL, Hu TM, Morrison JR. HPRT gene knock-out from rat  fetal neural stem cells. Yi Chuan Xue Bao. 2004 Jan; 31(1):51-6.

    Li XL, Hu T,Su H,Chen M,Yu H,Morrison J. Isolation and  culture of rat fetal neural stem cells. Acta Scientiarum Naturalium  Universitatis Nei Mongol. 2004.35:540-545.

    Li XL. Type VI intermediate filament protein-Nestin.  Chinese Journal of Cell Biology.2003, 25(1)

    Li XL, Hu T, Zhalagahu, Yu H, Morrison JR. Studies of  effects of lipid on rat fetal neural stem cells. Yi Chuan. 2002, Nov; 24  (6):663-6.

    Li XL, Hu T. The constructions of rat HPRT gene knock  out vector. Acta Scientiarum Naturalium Universitatis Nei Mongol.2002, 33  (6):665-668.

   Li XL, Morrison JR, Zhalagahu, Hu T. “Effects of  NeurobasalTM-A and DMEM/F12 media on the growth of rat fetal neural stem cells.”  Acta Scientiarum Naturalium Universitatis Nei Mongol.2001, 32:661-663.

    Li XL, Guo J, Yu H, Wang D, Zhang Z. “Study on  amplification of BLG 5’-flanking and regulatory sequence by PCR.” Acta  Scientiarum Naturalium Universitatis Nei Mongol.2001, 32: 79-82.

    Guo J, Yu H ,Li XL, Wang D, Zhang Z. “Study on  production of transgenic mice expressing human CD14 in mammary gland.” Acta  Scientiarum Naturalium Universitatis Nei Mongol.1997, 29: 223-228.

    Guo J, Li XL, Yu H, Wang D, Zhang Z. “Study on  construction of a non-milk protein fusion gene by mammary-specific expression  from PCR.” Acta Scientiarum Naturalium Universitatis Nei Mongol.1997,  28:830-831.

    Li XL, Shi Y, Guo J, Yu H, Wang D, Zhang Z. “Isolation  and cloning and sequence analysis of regulatory sequence of sheep  α-lactoglobulin gene .” Acta Scientiarum Naturalium Universitatis Nei  Mongol.1996, 27:371-377.

    Yu H, Shi Y, Guo J,